FastQCFastQC Report
Thu 26 May 2016
SRR1781434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406915
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT14394735.375201209097725No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG132833.2643181008318694No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC70911.7426243810132338No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT34560.8493174250150523No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC23360.5740756669083223No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT16430.4037698290797832TruSeq Adapter, Index 11 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13510.33201037071624295No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC12070.2966221446739491No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG8810.21650713293931168No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT7850.19291498224444908RNA PCR Primer, Index 38 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6890.16932283154958652No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG6370.15654374992320264No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT6280.15433198579555926No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC5440.13368885393755453No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT4930.12115552388090879No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC4730.11624049248614575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA150.002219102370.0378737
GCTTGCG150.002224519269.99483546
TATCCCG150.002224519269.99483543
CCGTTCT150.002225603769.9862414
AGGGGGG176800.066.8276570
ACTCGTA750.065.3686838
TGCCGAC650.064.6106245
CCGACTT453.8562575E-1062.21763247
CGACTTC453.8562575E-1062.21763248
CGTATTC850.061.7601548
AAGGGGG192050.061.5499969
CCGTCTA352.9726834E-759.9955847
ATGCCGA700.059.9955844
ATGACGT1600.059.05814444
CATATAC7900.058.498271
AAAGGGG202450.058.37801468
TCGTAAG1500.058.3648940
AACTCGT900.058.3648937
AAGCCGT900.058.3290344
AAAAGGG203900.058.0143667
Produced by FastQC (version 0.11.5)