FastQCFastQC Report
Thu 26 May 2016
SRR1781433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254010
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17403568.51501909373646No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG8330.32793984488799655No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGG207.928884E-569.9783218
CGATACT207.928884E-569.9783223
CCCACGT150.002225633769.9783123
ATCCGAA150.002225633769.9783121
AACGGAA150.002225633769.9783122
CTGCCGG301.0232543E-769.9783118
AGATTGC150.002225633769.9783118
ATACACG150.002232603569.923184
TGCGGGT308.396328E-658.292285
ACTGCTA252.3911311E-455.98265529
ACGATAC252.3911311E-455.98265522
ATACGAT252.3911311E-455.98265520
GTGGTCG1700.055.57101448
CGTATCA1400.055.04807356
CGCCGTA1500.053.6606253
ATCTCGG1500.053.6394742
TCTCGGT1500.053.6394743
CCGTATC1450.053.13937855
TGGTCGC1650.053.01387849
TCATTAA1400.052.5770260
Produced by FastQC (version 0.11.5)