FastQCFastQC Report
Thu 26 May 2016
SRR1781433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254010
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT13475253.04987992598716No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG118864.679343332939648No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC66682.6250935002558955No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT33301.3109720089760246No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC19270.7586315499389787No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT13970.5499783473091611TruSeq Adapter, Index 9 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10130.3988031967245384No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC9930.39092949096492263No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG7310.28778394551395614No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT6480.2551080666115507No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5110.20117318215818275No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4510.17755206487933545No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4440.17479626786346994No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3770.14841935356875713No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG3170.12479823628990985No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC3040.11968032754615959RNA PCR Primer, Index 42 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT2790.1098381953466399No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA301.0185249E-770.02521534
TCGGAAC600.070.02521533
ACGGAAC207.902634E-570.02521533
TAAGCCC207.902634E-570.02521543
TAAGACG301.0185249E-770.02521543
CGGAAAT353.6870915E-970.0252134
ATTCGAT500.070.0252131
CGTAAGA252.8285613E-670.0252141
TTCGATC500.070.0252132
AAGACGA150.002221464970.0114344
AAGCCGA150.002221464970.0114344
TATTCGA600.070.0114330
CGAAATC500.070.0114228
ATGCCGA700.070.0114244
GTCTAAA207.918069E-569.9976449
AGTCTAA207.918069E-569.9976448
TGCCGAC650.069.9976445
TATCCGA600.069.99763511
ATATCCG650.069.98386410
ATACGCA207.925796E-569.9838644