FastQCFastQC Report
Thu 26 May 2016
SRR1781432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273498
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16284559.54156885973572No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG9570.3499111510870281No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGT150.002210688570.0987266
GTAGTAT150.002228441669.957526
CGTGTGC252.3993096E-455.9455221
CGCCGTA1150.054.79953853
GTATCAT1200.052.54511657
ATCGTGC200.006953005752.4873610
GGACTCA200.00695804552.47774534
TAGTATC200.00695804552.4777457
TGCGCAT200.006963087252.4681365
TGCTATC200.006963087252.4681366
GCATAAA200.006973179552.4489251
TGTGCGC407.5324715E-752.4297333
CGTATCA1250.050.4433156
CCGTATC1250.050.4433155
GCCGTAT1250.050.43406354
TCGCCGT1250.050.40633452
CGCGGGC352.0794487E-550.0154516
TGCCGGC707.2759576E-1249.99712419
GTGGTCG1600.048.11341548
ACACGAG305.8625446E-446.68108716
Produced by FastQC (version 0.11.5)