FastQCFastQC Report
Thu 26 May 2016
SRR1781432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273498
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT12400245.3392712195336No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG117604.2998486277779No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC63382.31738440500479No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT31861.1649079700765637No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC17720.6479023612604113No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT12220.4468039985667171TruSeq Adapter, Index 2 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12070.44131949776598No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC8940.32687624772393215No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC7180.26252477166195No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT6890.2519214034471916No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG6660.24351183555272798No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG4700.17184769175642967No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC4210.1539316558073551No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC4110.150275321940197No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3680.13455308631141727No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT2990.10932438262802655No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA207.910556E-570.0129938
CACGAAT150.002221450470.0129939
AACTCGA301.0200529E-770.0129937
CGGTAAT150.002221450470.0129928
ACTCGTA500.070.01298538
TGACGTA150.002223062870.0001845
CCGTCTA550.070.0001847
CCGTCAA150.002223062870.0001847
CTACACG150.002223062870.000184
CGTCAAC150.002223062870.0001848
CCGATCC150.00222467669.9873914
GACCGGA150.00222467669.9873926
CGACACG150.00222467669.9873923
ACTTTCG150.00222467669.9873924
CCACGAC150.00222467669.9873920
CGATCCC207.924903E-569.9873815
AGGGGGG152350.067.7717670
TCTTTAT2350.065.532081
AAACTCG700.065.0239636
GAATCAC650.064.639235
Produced by FastQC (version 0.11.5)