FastQCFastQC Report
Thu 26 May 2016
SRR1781431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342681
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19367756.51816120531923No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG9210.2687630770308246No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC150.00222693869.9732530
TATGACC200.006918248352.55676364
CGTAACT200.00693428552.5260155
GACGCGC200.00693829952.51832617
CTTCGGG200.006954370552.4876148
GACTCAT200.00695839352.479947
TACGCGG200.006966442352.46465
ATACGCG200.0069704752.4569364
GCCGTAT1150.048.71977254
GAATTAT305.846587E-446.71028560
CGTATCA1200.046.69661356
TGCGGAC305.8677135E-446.67612519
TCGCCGT1150.045.66810252
CCGTATC1250.044.8221955
CGCCGTA1200.043.7716753
TCGGTGG1800.040.81176445
CATTAAA1300.040.4282861
AACTGTG350.001256892739.9847157
GTGGTCG1500.039.65730748
TGGTCGC1500.039.65730749
Produced by FastQC (version 0.11.5)