FastQCFastQC Report
Thu 26 May 2016
SRR1781431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342681
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT14716242.94431264061912Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG132563.8683206830842676No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC69712.0342534310335267No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT36871.0759277578856137Illumina PCR Primer Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC22580.6589218544360499No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT13460.3927851266921714Illumina PCR Primer Index 6 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11470.33471362579191727No Hit
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC10360.3023219845862478Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG7970.23257782018845513No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT7540.22002970692860122Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT5010.14620011030667004TruSeq Adapter, Index 4 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG5010.14620011030667004No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC4930.14386557760716234Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT4750.13861287903327TruSeq Adapter, Index 6 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC4280.12489749942366224Illumina PCR Primer Index 6 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3960.11555936862563143Illumina PCR Primer Index 6 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT3640.1062212378276006Illumina PCR Primer Index 6 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATG207.911851E-570.0157740
ATGCGTT150.002224156469.995324
TAAGCGG150.002224156469.9953246
TCCGATC252.842784E-669.974913
ATCAACG252.842784E-669.974910
ATATACG150.002226733169.974910
CCGAAAC150.002226733169.974914
TATACGA150.002226733169.974911
TCAACGA252.842784E-669.974911
AGGGGGG177700.067.6812270
ACTCGTA1300.064.6488138
AATGCCG1050.063.33835243
TATCCGA1050.063.3106211
AACTCGT1350.062.25441437
GACAACG453.8562575E-1062.19991318
TCGAATG1300.061.93702740
ATGCCGA850.061.76960444
AAGGGGG194950.061.73735469
GCAAACG401.0672011E-861.22804318
CTCGAAT1150.060.89216239
Produced by FastQC (version 0.11.5)