FastQCFastQC Report
Thu 26 May 2016
SRR1781430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304406
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17419257.223576407823764No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG10040.3298226710380216No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGA150.002220860470.01958511
CGCTGGC150.002222310570.00806416
GCGCGTC252.8479044E-669.950526
CGCGTCT207.947044E-569.9505167
GTTGGAC150.002232480369.9275341
TTGTGCG352.9933835E-759.9280322
CCGACGG252.3868709E-456.00645421
CTGAACG252.3965909E-455.96041526
CGTAGCA200.00691690552.5579356
TACGGTG200.006939461552.51468714
ACGTAGG200.006939461552.51468717
TACGTAG200.006943979352.50604616
TTACTTG200.006953021552.48877350
CGACGGG200.00695754652.4801422
CGACGAG200.00695754652.4801422
ATCGATA200.006962072652.47151623
CATTGCT200.00696660152.46288727
TATGCGC200.00697566552.4456443
GGTGTTA200.00698473852.428411
TGTGCGC352.0966301E-549.9482353
Produced by FastQC (version 0.11.5)