FastQCFastQC Report
Thu 26 May 2016
SRR1781430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304406
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT13399144.01720071220672No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG127544.189799149819649No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC63332.080445194904174No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT34431.13105523544214No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC18710.6146396588766319No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT13370.4392160469898754RNA PCR Primer, Index 37 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12300.4040656228852257No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC9810.32226697239870433No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG7010.2302845541809294No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6960.22864201099846915No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5150.1691819477934075No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC4220.13863064459964652No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC3750.12319073868452002No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3450.11333547958975843No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACG150.002222439370.00706536
ACGTAAG150.002222439370.00706540
CACGTAA252.8342747E-670.00706539
CTCTCGT150.002222439370.00706537
TGCGTTT150.002222439370.00706541
GGCGTTC207.921758E-569.9955749
TAACCGA353.7034624E-969.9955611
CTGTTAC150.00222388869.9955619
CGACTAC150.00222388869.9955648
GAACACG353.7034624E-969.9955618
ATGCCGA700.069.9955644
AGGGGGG160900.068.2337170
CCGTATT850.065.87817447
TCTCGAA750.065.3399338
ACCGAGC1400.064.9958813
AACCGAG600.064.162612
CGTAAGC1050.063.33972541
ACCACGA1550.063.22179819
AAGGGGG176100.062.32427669
ATGGGAC401.0628355E-861.25617629
Produced by FastQC (version 0.11.5)