FastQCFastQC Report
Thu 26 May 2016
SRR1781429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213278
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18419486.36333799079136No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG9980.4679338703476214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGACG150.002220281970.0169111
AGACGCA150.002220281970.0169113
GTAAACC150.002224421169.9840423
ACACTGT150.00222856669.951216
GTATCAT1200.058.3885557
CGTATCA1150.057.8808256
GTGGTCG1400.057.4868948
CCGTATC1100.057.3134755
GCCGTAT1100.057.3134754
CGCCGTA1100.057.28655653
TCTCGGT1500.055.96096443
TAGATCT1500.055.96096439
TATCATT1200.055.4821658
TGGTCGC1200.055.41703849
GTAGATC1400.054.96166238
TCGCCGT1150.054.79583752
GGTCGCC1150.054.79583750
CTCGGTG1550.054.15577344
CATTAAA1300.053.9097961
CGGTGGT1500.053.6418446
Produced by FastQC (version 0.11.5)