FastQCFastQC Report
Thu 26 May 2016
SRR1781429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213278
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13913165.23457646827146No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG128336.017029417005036No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC68833.2272433162351484No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT32541.5257082305723046No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC22991.0779358396083984No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT14250.6681420493440486TruSeq Adapter, Index 7 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12340.5785875711512674No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT9710.45527433678110263No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC9580.44917900580463055No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG8150.38213036506343834No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT7110.33336771725166214No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT6210.29116927202993276No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT6080.28507394105346073No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5700.26725681973761944No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC5230.24521985389960524No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC4180.19598833447425426No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3860.1809844428398616No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG3210.15050778795750147No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT3180.14910117311677717No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC2970.13925486923170696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA150.002221704270.0056339
GTATGAA353.6925485E-970.0056342
CAGTCTA150.002221704270.0056347
CCAACAC150.002221704270.0056335
ATCTCGA600.070.0056337
AAATCGT150.002221704270.0056337
AACGAAC207.9106874E-570.0056330
ACGTCAT150.002221704270.0056347
AATCACG207.9106874E-570.0056336
CACGTAT750.070.0056339
TCGAAAG150.002221704270.0056340
ACACCGA150.002221704270.0056311
CACGAAT207.9106874E-570.0056339
TCAACGA353.6925485E-970.0056311
ACCGAGA207.9106874E-570.0056313
GCAGTAT301.01972546E-770.0056346
TCACGAA150.002221704270.0056338
AAAGGGT207.9106874E-570.0056368
CGTATCA150.002221704270.0056341
ATAGACC353.6925485E-970.0056330
Produced by FastQC (version 0.11.5)