FastQCFastQC Report
Thu 26 May 2016
SRR1781428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392820
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17545544.66549564686116No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG11060.2815538923680057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGT150.002226411869.9793630
CGACGCA200.00694623952.5045948
TTCGTCA200.00694623952.5045951
CGCTCTC200.00694623952.5045915
ATCCCGC200.00694623952.5045916
ACGCTGG200.00694623952.5045915
CGACGGG200.00694974252.497922
GCGACGC200.00695324652.49120747
GCGACAG200.006956751552.48451625
AGTATGC352.0760157E-550.03626362
TCCGGCG350.001256579539.98820525
TGCGCGG350.001258943739.9729235
CCGTATC2450.038.58955855
GCCGTAT2450.038.58463754
TCGCCGT2500.037.80330752
CGCCGTA2500.037.80330753
TGGTCGC2700.037.5958849
CGTATCA2600.036.36323556
GTCGCCG2800.036.25316651
GTGGTCG2850.035.61714648
Produced by FastQC (version 0.11.5)