FastQCFastQC Report
Thu 26 May 2016
SRR1781428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392820
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT13317533.90229621709689No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG129213.289292805865282No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC75911.9324372486125962No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT31770.8087673743699404No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC18210.4635711012677562No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT14740.37523547680871644RNA PCR Primer, Index 36 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13930.3546153454508426No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC9950.25329667532203043No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT7160.18227177842268724No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT7050.1794715136703834No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG7020.17870780510157322No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG4620.11761111959676188No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4590.11684741102795174No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT4140.1053917824957996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC150.002221870570.0154131
AGGGGGG157350.067.7424570
ATATCCG550.063.62606410
TATCCGA550.063.62606411
ACGGTAC453.8380676E-1062.22007827
AAGGGGG173450.061.45445369
AATATCG401.0628355E-861.26348536
AATGCCG1150.060.87521443
CAAATCG352.9663715E-760.01320336
TATGACG1400.060.00556643
ACGTCTT1350.059.63516247
TCGAATG1300.059.2438140
AACTCGA308.358951E-658.34617237
ATCCCGT308.365265E-658.3387444
AAAGGGG182900.058.31751368
AAAAGGG183250.058.2634267
AAAAAGG184250.058.1065466
AAAAAAG185650.058.0076965
CGTCTTC178500.057.09606648
CCGTCTT177950.057.06345447
Produced by FastQC (version 0.11.5)