FastQCFastQC Report
Thu 26 May 2016
SRR1781427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295613
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19731966.74909425498879No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG11160.37752060971608153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC207.911657E-570.0128811
TAAGGTT150.0022216470.0128811
GAACGCA150.002223133670.00101549
TGGACAA207.9249505E-569.9891618
TTATGCG150.00223061469.9417652
CGCCGTA1250.056.01030353
GTGGTCG1600.054.6790348
CTCGGTG1550.054.1759844
TCGGTGG1650.053.0220945
CGTCTGT200.006918654352.55418865
GTATCAT1400.052.5363757
CGTCCGA200.00694654552.50076310
TAGACTC200.006951201752.49186725
GCATTGT200.006955860652.482981
TCTCGGT1600.052.4740943
TAGGGCG200.00696985152.4563239
TAGAGCG200.00697451952.4474455
TATGCGC200.00697451952.4474453
TACATAG200.00697451952.4474455
TACGCGT200.00697451952.4474455
Produced by FastQC (version 0.11.5)