FastQCFastQC Report
Thu 26 May 2016
SRR1781427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295613
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT15068650.97407759469306No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG142774.829625219459226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC73182.4755338905934448No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT37491.2682121557576966No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC24150.8169464807028107No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT15460.5229810596962922RNA PCR Primer, Index 36 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13930.47122420191263575No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC13340.45126567505488596No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG9830.33252935425708613No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT7930.26825613217280697No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6760.2286773585735404No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC6470.218867235202782RNA PCR Primer, Index 36 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC5280.17861190137104932No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4300.14546044998021063No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG3950.13362064591205391No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC3660.12381052254129554No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT3580.12110428161143116RNA PCR Primer, Index 36 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC3110.10520511614847791No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT2970.10046919452121524No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC150.002218622570.0368961
TAACGTA207.911497E-570.013238
ATCGTAA150.002221603270.013239
CGAATGA252.8325958E-670.013241
CGTAAGA301.0203257E-770.013241
TCGAAAG353.6961865E-970.013240
ACGACAA207.911497E-570.013231
CGTCTAA207.918131E-570.0013648
AAGCCGT800.070.0013644
CCGTCTA750.070.0013547
ATTCCGT150.002223094770.0013544
CGTAATC150.002223094770.0013548
ACGTAAT150.002223094770.0013547
CGACTTA150.002223094770.0013548
TAAGACG301.02152626E-770.0013543
CGAGACG207.924769E-569.9895223
AGACGTC150.00222458769.9895145
ATACGAG252.8382965E-669.9895112
GAACACG252.8382965E-669.9895118
CACCGAA150.00222458769.9895112
Produced by FastQC (version 0.11.5)