FastQCFastQC Report
Thu 26 May 2016
SRR1781426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221644
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19721488.97782028839039No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG10380.46831856490588514No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2260.10196531374636805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA150.002214734470.0619763
CGCTGGC150.00221871270.03030416
GGGCGCT207.8975456E-570.030313
GGCGCCG150.002220702670.0144751
CATCTGG150.002220702670.014479
CCGACGG150.002220702670.0144721
GGTCGGC150.002220702670.0144750
GGCCGGC207.906396E-570.01446519
TGGCGGC252.8299364E-670.01446519
ATCTGGC252.8299364E-670.01446510
TACTAAG150.002222694569.9986528
CGCATCT150.002226682669.9670267
GCGCATC207.932996E-569.967026
TGCGCAT150.002228678769.951225
TCGGTGT150.002228678769.9512233
CGGTGTA150.002228678769.9512234
GTTATGC150.002228678769.951221
TTATGCG150.002232674769.919642
TTGTGCG252.8528193E-669.919642
GGCGCTG700.065.0281414
Produced by FastQC (version 0.11.5)