FastQCFastQC Report
Thu 26 May 2016
SRR1781426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221644
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT14980967.58991896915775Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG133366.016855858944975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC64942.9299236613668764No Hit
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT35381.5962534514807531Illumina PCR Primer Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC23751.0715381422461245No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT15260.6884914547652993Illumina PCR Primer Index 1 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC12350.5571998339679847Illumina PCR Primer Index 1 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12210.5508833986031654No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG9940.44846691090216745No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT9040.4078612549854722TruSeq Adapter, Index 1 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT7460.3365757701539406Illumina PCR Primer Index 1 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG6360.28694663514464636No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC5670.25581563227518006Illumina PCR Primer Index 1 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC5120.23100106477053295Illumina PCR Primer Index 1 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4180.1885907130353179Illumina PCR Primer Index 1 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG3610.1628737976214109No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT3370.15204562271029218No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3240.14618036130010287Illumina PCR Primer Index 1 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT3220.14527801339084298Illumina PCR Primer Index 1 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC3060.13805923011676383No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2740.1236216635686055Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT2510.11324466261211673No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT2380.10737940120192742TruSeq Adapter, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAT150.00221863670.0309432
AATCTCG150.00221863670.0309436
TAACGTA301.0173608E-770.0309438
ATAACGT301.0173608E-770.0309437
AACCATC150.00221863670.0309433
TATCTCG353.6816346E-970.0309436
AAGATAT353.6816346E-970.0309434
CGAACAC301.0173608E-770.0309435
AATATCT150.00221863670.0309434
ATATCTC150.00221863670.0309435
ACACGTA207.897214E-570.0309338
TCAAGTA207.897214E-570.0309338
AACGAAC207.897214E-570.0309333
CACGTAA252.8259965E-670.0309339
GATCAAG207.897214E-570.0309336
ATCGTAA252.8259965E-670.0309339
AGATATC207.897214E-570.0309335
AACACGT207.897214E-570.0309337
CTATCTC252.8259965E-670.0309335
CGATCAA207.897214E-570.0309335
Produced by FastQC (version 0.11.5)