FastQCFastQC Report
Thu 26 May 2016
SRR1781425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352685
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17351049.19687539872691No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG10460.29658193572167796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGT150.002224979769.9892124
GTTCGTA150.002224979769.9892125
CCGTTCG150.002224979769.9892123
GCGCGTC252.3939289E-455.975486
TCCGTTC200.00695229152.4919122
ACCGCTA200.00695619552.484468
TTGAACG200.006960100552.4770140
TGCGCGT352.0934207E-549.963935
GCGTCAG352.0951784E-549.9568371
CGTCAGA352.0951784E-549.9568372
TTGTGCG305.898882E-446.6263852
GTGGTCG1600.041.55609548
CGTATCA1450.041.0456456
CCGTATC1450.041.0456455
TCGCCGT1550.040.65620852
TGGTCGC1650.040.3025449
CGCGTCT350.001256411539.988167
GTCGCCG1600.039.38570451
CGCCGTA1600.039.38570453
TCTGGCG753.5217454E-837.34348311
Produced by FastQC (version 0.11.5)