FastQCFastQC Report
Thu 26 May 2016
SRR1781425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352685
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT13194237.410720614712844No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG128623.646880360661781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC66971.898861590371011No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT32520.9220692686108No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC18540.5256815571969321No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13060.3703021109488637No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT10660.3022527184314615TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7090.2010292470618257No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT6980.19791031657144476No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT6520.1848675163389427No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG6450.18288274239051844No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG5970.16927286388703802No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT4210.11936997604094304No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3940.11171441938273531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC207.912745E-570.0147556
TAGACGC207.912745E-570.0147554
AATTCGC150.002221764570.014755
ATCGAGT150.002221764570.0147537
AGGGGGG158300.067.5408870
ACTCGTA800.065.6388338
ATGCCGA900.062.2265144
AAGGGGG172800.061.93414369
GCCGACA401.0648364E-861.24553746
CATATAC7050.061.0853581
TGCCGAC750.060.66224345
AAGCCGT1100.060.4587144
AAAGGGG181000.059.22496468
AAAAGGG181600.059.04855767
AAAAAGG182900.058.705466
AAAAAAG183950.058.65568565
TATCTCG176350.058.26291336
CCGTCTT173750.058.21158247
CGTCTTC174450.058.1786248
TATGCCG175550.058.1612343
Produced by FastQC (version 0.11.5)