FastQCFastQC Report
Thu 26 May 2016
SRR1781424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228256
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17161675.18575634375438No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG9370.41050399551380906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTAG150.002219495170.0247756
GGTGCCG207.918338E-569.9940917
GCGGCGA150.002223358569.9940921
TGCCGGC401.3460522E-1069.9940919
CCGGCGG455.456968E-1269.9940821
GCCGGCG455.456968E-1269.9940820
CTGCCGG455.456968E-1269.9940818
AAGTATG150.002225292169.97874524
CGACGAA150.002225292169.97874522
TGCGCGT150.002225292169.9787455
TGGCGCT308.351999E-658.34118713
GGCGCTG452.6879206E-854.45177514
CAAGTAT200.006941233352.50707213
GGCTGCC200.006941233352.50707216
TCTGGCG200.006941233352.50707211
TGGCGGC200.006947254352.49556419
TCTTACC200.006947254352.4955648
GTATGCA200.006947254352.4955642
TTAACGG200.006947254352.49556450
TGGGCGC200.006947254352.49556449
Produced by FastQC (version 0.11.5)