FastQCFastQC Report
Thu 26 May 2016
SRR1781424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228256
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT13328158.39101710360297No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG125095.480250245338567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC57192.5055201177625124No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT35361.5491378101780457No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC18240.7991027618112996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11360.4976868077947567No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT9830.43065680639282206Illumina PCR Primer Index 6 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8240.3609981774849292No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC8210.35968386373195005No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT7390.3237592878171877No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5810.2545387634936212No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5360.23482405719893454No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC4070.17830856582083274No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3870.16954647413430535No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC3360.14720314033366044No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT2760.12091686527407823No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2560.11215477358755081No Hit
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG2390.10470699565400252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC150.002222614170.000013
TCCGAAC150.002222614170.0000113
ACCGAGA150.002222614170.0000113
TTGTACA150.002222614170.000012
ATCACGA150.002222614170.0000137
AAAACGG301.02061676E-770.0000167
ACGGGGG301.02061676E-770.0000170
CCAGACG150.002222614170.0000123
CTGAATG150.002222614170.0000154
AACGGGG252.8337E-670.0000169
AAACGGG252.8337E-670.0000168
TACGAGA252.8337E-670.0000113
ACTCGAA150.002222614170.0000138
TCTCCGG150.002222614170.0000111
CTCGAAA301.02061676E-770.0000139
AACTCGA150.002222614170.0000137
AACTCCG550.070.0000110
GAACCCA150.002222614170.0000116
ATACGAG301.02061676E-770.0000112
CTGATTG500.070.0000154
Produced by FastQC (version 0.11.5)