FastQCFastQC Report
Thu 26 May 2016
SRR1781423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304096
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18411160.54370988109018No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG10260.3373934546985163No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAA150.002225046369.9863435
GGACTAT200.006934413752.52433856
ACCGGAC200.006947977552.49839414
GAGTATC200.006947977552.49839449
CCGGACC200.006947977552.49839415
GGACGAA200.006947977552.49839422
CGAGACA200.006947977552.49839416
CACAAGC200.006947977552.49839412
GTCAACC200.00695250352.4897548
TCCGTTT200.00695250352.4897546
CTGTCAA200.006970627252.4552152
TATACGC305.8829616E-446.649883
CGTATCA2150.043.9738656
CCGTATC2150.043.96662555
ACGAAGA404.587613E-543.7486624
CGCCGTA2100.043.33200553
GCGGGTG651.0000804E-843.0685166
TCGCCGT2100.041.6653952
TCATTAA2200.041.38281260
GCCGTAT2200.041.37599654
Produced by FastQC (version 0.11.5)