FastQCFastQC Report
Thu 26 May 2016
SRR1781423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304096
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT14117346.42382668630959No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG133684.39598021677365No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC67182.2091707881721563No Hit
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT31081.0220456697884877No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC19860.6530832368725665No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT12960.4261812059349679Illumina PCR Primer Index 7 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12120.3985583499947385No Hit
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC9330.3068110070504051No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG7150.23512311901504787No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT6240.2051983584131327No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5410.17790434599600127No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC4670.15356992528675156No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3970.13055087866989373No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC3450.11345101546879933No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT3170.10424339682205619No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG150.002219060770.033939
ACTCGAA401.3460522E-1070.033938
CATAACG252.827821E-670.033936
AACGCAC150.002220509670.0223730
CGTAGGC150.002221959270.0108641
TCGAAAG207.913172E-570.0108640
TATCACG150.002223409569.99934443
AAGCCGT1350.069.99934444
AGGCCGT150.002223409569.99934444
TAATCCG150.002223409569.99934443
TCCGATC252.8388968E-669.9878313
CATGACG207.926078E-569.9878317
TACGAGA207.926078E-569.9878313
AGGGGGG165700.067.97159670
TAAGCCG1400.067.4993743
CGGACAT1150.066.98894532
CATCACG1550.065.5155936
TCGTAAG1500.065.3434740
CACGTAT1300.064.64667539
CATATAC6850.064.389551
Produced by FastQC (version 0.11.5)