FastQCFastQC Report
Thu 26 May 2016
SRR1781422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401264
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22418555.86970174249372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG150.002216341270.0596765
TCCGCGA150.002219635370.0334454
GGATCCG150.00222073470.02470411
CCACCGA252.3953023E-455.9708756
TCCGATT200.006925374352.5447556
ATCCGGA200.00695619852.48578626
TCGATGT200.006959629252.47924431
ACGTCAG200.006959629252.47924428
CGCTTCT200.006959629252.47924431
GGTACTA200.00696306152.4727021
CTTAACG200.00696649552.466164
CGGTCGC305.8609794E-446.6889615
CACCGAG305.8862614E-446.6482167
TGGTGCG305.8935006E-446.636592
CTGCCGG503.472731E-642.0043418
GTCGCCA350.001251846840.01910817
AGCGGTC350.001252615240.01411413
GTGGTCG801.4242687E-939.36433848
TGGTCGC852.57387E-937.0534149
CCGTATC400.002408950435.02546355
Produced by FastQC (version 0.11.5)