FastQCFastQC Report
Thu 26 May 2016
SRR1781422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401264
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT17298143.10902547948483No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG136093.391532756489493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC78941.9672833845049642No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC24420.6085768970054627No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT17470.435374217472786TruSeq Adapter, Index 11 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT17450.4348757924957135No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC12360.3080266358307748No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT9950.2479664260935444No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT9770.24348060129989235No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG9240.23027233940747238No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT8910.22204832728577695RNA PCR Primer, Index 38 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC6380.15899756768611187No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG6180.1540133179153874No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT6130.15276725547270625No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC4700.11712986961202601No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4470.1113979823756928No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4090.10192790781131623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC150.002220095670.029791
TAGCGAA150.00222339170.0036230
ACTCGTA1350.070.0036138
CTCGGTC207.9252015E-569.99489612
CGTCTAA252.838853E-669.9948948
TCGCGGA150.002224490369.9948955
AATCGGA150.002224490369.9948950
AACTCGT1450.067.5896937
AGGGGGG200550.067.58668570
CGACTTC800.065.6202248
ATATCGT750.065.336737
TATCGTA750.065.336738
TAACTCG1450.065.1757736
CCGTCTA900.062.21767447
TCGTAAG2550.061.7678940
AAGGGGG220450.061.4538969
CTCGTAA2300.060.87270439
ATCGTAA352.9699913E-760.00309839
TATAACT1750.060.00309834
CGTATGA2450.058.5744541
Produced by FastQC (version 0.11.5)