FastQCFastQC Report
Thu 26 May 2016
SRR1781421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391973
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19840950.618027262081824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAA150.00222444969.99489646
CGATGAA200.00692958852.53643856
TTGCGTT200.006933092652.5297218
TTATACG200.006982295352.435882
CGGCCGG404.5739172E-543.7747718
GTGCGCG602.6111047E-740.799084
GCGCTGC953.6379788E-1240.54925515
GGGCGCT556.6619596E-638.20343413
TGTGCGG556.7265373E-638.149793
CGCTGCG654.837657E-737.71364216
GCCGGCG601.211921E-535.01086420
TCGCCGT751.4834222E-632.6809852
GGTCGCC751.4863872E-632.67262650
GTGGTCG1001.2536475E-831.50574948
TGCGTCT450.004293116731.12872314
CTTCACG450.004298491431.12076622
GCACTGA450.004311951331.1008956
GGTGCGC450.004322743531.0850123
GGCGCTG1251.03682396E-1030.81743814
CCGTATC703.4715835E-530.01698355
Produced by FastQC (version 0.11.5)