FastQCFastQC Report
Thu 26 May 2016
SRR1781421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391973
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT15756540.19792179563388No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG121313.094856023246499No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC71731.8299729828330014No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC21030.5365165457824901No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT17460.4454388440020104No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT15650.3992621940796943TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC10440.2663448757950165No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT10060.2566503305074584No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG8250.21047368058514238No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC5930.15128593040847202No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5390.13750947131562632No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4980.12704956718957683No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4600.11735502190201876No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCG150.002223100370.00561531
TACACGT150.002223100370.0056155
AGCGATT150.002224225669.9966823
AGGGGGG180650.067.8462270
ATTCGAT501.4551915E-1163.0050531
AAGGGGG196050.062.57036269
ATGCCGA900.062.22721544
TGCCGAC900.062.22721545
CCGACTT850.061.76177647
AAGCCGT750.060.6715344
GTATACG750.060.6715329
CTCTCGT352.9692274E-760.0048137
AAAGGGG206500.059.5395568
AAAAGGG207350.059.48114867
AAAAAAG209300.059.21124365
AAAAAGG209050.059.11463566
CGTCTTC203350.058.79239748
CTCGTAT204350.058.7690939
TGCTTGA204700.058.76370255
TATGCCG202850.058.7550243
Produced by FastQC (version 0.11.5)