FastQCFastQC Report
Thu 26 May 2016
SRR1781420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325925
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20101261.6743115747488No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCG150.002222129670.0106151
ATACACG150.00223299369.924594
CCGTATC401.060107E-861.27812655
TGGTCGG401.0639269E-861.24986349
GACGAAG352.9693365E-759.99986623
CGTATCA509.731593E-1056.03433656
TTTGCGT200.0069181152.55644661
GACATTA200.006922322352.54836359
TCCGTTT200.00692653752.5402757
CTCCGTT200.006930753652.53219256
CGGGGTA200.00693497252.5241153
CGCCGTA407.4439777E-752.5241153
TCGTCCG200.006939192352.51603315
GTCCGAC200.006939192352.51603317
CGTCCGA200.006939192352.51603316
CGACGAG200.006943414552.50795722
ACACGTC200.006956092552.4837466
ACGCGTC200.006956092552.4837466
TTGTGTC200.006973023552.451537
TGGACGA200.006973023552.45155
Produced by FastQC (version 0.11.5)