FastQCFastQC Report
Thu 26 May 2016
SRR1781420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325925
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT15821748.54399018179029No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG123593.7919766817519367No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC73742.2624837002377847No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC19420.5958426018255734No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT17750.5446038198972156No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT15840.4860013806857406TruSeq Adapter, Index 2 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT11390.34946690189460766No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC10720.3289100253125719No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC9020.2767507862238245No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG7210.22121653754698165No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC5290.16230727928204342No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG5190.15923908874741122No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT4650.14267085986039732No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC4330.1328526501495743No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3810.11689805936948684No Hit
ACTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG3560.10922758303290635No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG3520.10800030681905345No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT3310.10155710669632584No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT3260.10002301142900973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG150.00222134470.01688436
AATCCCG150.00222134470.01688443
ATCCCGT150.00222134470.01688444
TCGTATC150.00222134470.01688440
ACACTCG150.00222134470.01688436
ACGTAAG207.91065E-570.01688440
CACGTAA207.91065E-570.01688439
GCCGTAT455.456968E-1270.01688446
TGTATCG150.002224050269.995455
GTATCGT150.002224050269.995456
ACGATGT150.002225404169.9846668
CGATGTC150.002225404169.9846669
ACTCGTA1200.067.0995138
AGGGGGG185600.066.97835570
AGAGACG1450.065.15813423
TGCCGTA501.4551915E-1163.01519445
AACTCCG1450.062.75449810
CCGTATT453.8380676E-1062.22767647
CGACTTC1300.061.92850548
CGAATGC850.061.77960241
Produced by FastQC (version 0.11.5)