FastQCFastQC Report
Thu 26 May 2016
SRR1781419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354640
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18816853.058876607263706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTATG252.3914149E-455.9874737
TCGATGT252.3947524E-455.9716731
CGCCGTA452.689194E-854.46300553
CCGTATC407.451963E-752.517955
GCCGTAT407.451963E-752.517954
CGCAAGT200.006942601452.51048712
TAGCACG200.006950358452.49566350
CGGGTTC200.00695423952.4882527
AACCGAA200.00695423952.488257
CGTATCA352.0803256E-550.01704456
CGACGAA305.876262E-446.66280722
ACGCGAT305.8926357E-446.6364841
TGGTCGC551.310018E-744.5417749
TGTGCGG404.6097397E-543.7155343
CGCGTCG350.001255975139.9910477
GTCGCCG753.52411E-837.3407951
GTGGTCG753.5328412E-837.33024648
TCGCCGT601.2125161E-535.0069952
CTGCCGG806.2194886E-835.0069918
GGTCGCC806.23495E-834.9971150