FastQCFastQC Report
Thu 26 May 2016
SRR1781419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354640
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT14646041.298217911121135Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG115843.2664110083464926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC63361.7866004962779156No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC19750.55690277464471No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT16670.47005413940897817Illumina PCR Primer Index 6 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT14480.40830137604331157Illumina PCR Primer Index 6 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC9970.2811301601624182Illumina PCR Primer Index 6 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT9480.2673133318294609Illumina PCR Primer Index 6 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG7470.2106361380554929No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT5690.16044439431536206TruSeq Adapter, Index 6 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC5020.1415519963907061Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT4750.13393864200315814No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG4700.13252876156101964No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4140.11673810060906836Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT3900.10997067448680353TruSeq Adapter, Index 4 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3670.10348522445296639Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT3630.10235732009925558No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA150.00222247770.0091737
AATTCGG150.00222247770.0091736
ACTCGAA150.00222247770.0091738
ATAACGT207.915932E-570.0091737
CACGTAA301.021308E-770.0091739
TATAACG150.00222247770.0091736
GCCGTAA150.002223720869.999346
TCGAAAG500.069.999340
AGGGGGG170600.067.373370
CCGTCTA1150.063.912447
CGTCATC1900.062.6309548
AAGCGGG453.8380676E-1062.22159269
AACGGGG453.8380676E-1062.22159269
AAACGGG453.8380676E-1062.22159268
TATCCGA900.062.2128211
ATCCGAG1250.061.59069412
GACCGTC401.0644726E-861.24938226
AGACCGT401.065564E-861.24074625
AAGGGGG188100.061.19821569
AACTCGT1150.060.87753737
Produced by FastQC (version 0.11.5)