FastQCFastQC Report
Thu 26 May 2016
SRR1781418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392271
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19776950.41642130058046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGA150.00222234570.0116114
GTTATAG252.3886347E-456.002141
TGTCGGT252.3916483E-455.98785843
TCGCGCG200.00695459852.48861729
TTATGCG200.006961617652.475232
TTGTACG200.006961617652.475232
CGACGAA350.001254520540.0015322
CGTCTGG350.001254520540.001539
TGTGGCG400.00241552835.00580611
CCGTAAT400.00241552835.00580621
GGGCGCT904.485628E-935.00580613
CTGCCGG1201.8189894E-1235.00580618
TGCCGGG904.485628E-935.00580619
TAGGATG400.00242461534.9790274
ATATATC601.2205708E-534.9745643
TGGCGCT1350.033.70929313
GGCGCTG2200.031.82345814
TCGGTGG1109.058567E-1031.81128145
TGCGGGT552.9981733E-431.8031675
CGGTGGT1001.2585588E-831.4931746
Produced by FastQC (version 0.11.5)