FastQCFastQC Report
Thu 26 May 2016
SRR1781418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392271
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT15716840.06617873867811No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG122513.1230960229025344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC73151.864782255124646No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC18670.47594647577822474No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT16990.43311894073229984No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT13020.33191339660591784RNA PCR Primer, Index 37 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT11170.2847521228946315No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC10610.2704762778793232No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG7150.18227194974902552No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5820.14836681783766834No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC4680.11930527619936218No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC4290.10936316984941533No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4290.10936316984941533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACG207.907903E-570.02537536
TCGTATC207.9129E-570.0164540
CGTATCC207.9179E-570.0075241
ACCCGAA150.002225109869.9896819
AGGGGGG183850.067.8474370
ATCTCGA1350.067.4318537
CTCGAAT1450.062.78137239
ACTCCGA950.062.6223411
AACACGA453.8562575E-1062.2130419
ACTCGTA850.061.78710638
TCGTAAG1700.061.77922440
CGTAAGC1700.061.77134741
CCGTCTA850.061.7634747
AAGGGGG202000.061.7087269
CCGTCAT1250.061.59876647
CTAATAC3550.061.1489261
TAAGCCG1550.060.97429343
TCGAATG1500.060.68092340
CGAATGC1450.060.35131541
TCGAAAG352.9660623E-760.01410340