FastQCFastQC Report
Thu 26 May 2016
SRR1781417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357909
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16887147.18266374972409No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA150.002219930370.0295261
GTACGTG150.002229804669.951226
ATAGTAC150.002232278569.931673
ACGTCAA200.006944239652.5074521
CGACATT200.006951927752.49276425
TTATACG200.00696732352.4634132
AACGATT200.00696732352.46341337
GAACGAT200.00696732352.46341336
TACACGT200.00696732352.4634135
TATACGC200.0069750352.4487533
GGATAAG352.0793683E-550.021091
CCGACGG451.6808663E-646.6732921
CGACGAA451.682487E-646.6667622
GTGGTCG1450.043.4362148
CGTATCA1150.042.6445756
CGCCGTA1000.042.0177153
GCCGTAT1100.041.3926754
ATCTCGG1200.040.80487442
TCGCCGT1050.040.01127652
ACGAAGA350.001255459439.99448824
Produced by FastQC (version 0.11.5)