FastQCFastQC Report
Thu 26 May 2016
SRR1781417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357909
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13152736.74872663162983No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG104332.9149867703801804No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC56991.592304189053642No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC16670.4657608498249555No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13570.37914665459655944No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT12100.33807476202051356TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT10910.3048260870780003No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC8180.22854971515105793No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT8180.22854971515105793No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT7880.22016769625798735No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT7740.2162560874412211No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG6510.1818898099796317No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC4140.11567186072437408No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG3970.11092205001830074No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3960.11064264938853172No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG150.002220517370.024885
CACGAAT150.002222980470.0053139
ATCGCAC150.002222980470.0053130
CGTATCA150.002224212569.9955341
TGACGAA150.002224212569.9955345
AATGCCG750.069.9955343
ACGTATT150.002224212569.9955347
TCGGTAT150.002224212569.9955370
AATCGAA252.8380728E-669.9955329
AGGGGGG157700.068.17573570
ACTCGTA900.066.1161338
CACGTAT650.064.62028539
TCGAACA600.064.1715331
TCACGTA550.063.64119338
CGACTTC1100.063.632348
ATGCCGA1000.062.99597544
TCGAATG950.062.6363340
AAGGGGG172500.062.3467469
AACTCGT900.062.22694437
TGCCGAC900.062.21824645
Produced by FastQC (version 0.11.5)