FastQCFastQC Report
Thu 26 May 2016
SRR1781416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617613
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22135535.84040491375667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCA301.0189797E-770.0447856
CCGGATC207.9038204E-570.0390955
GCCGGAT207.9038204E-570.0390954
CGCCGGA207.910174E-570.0277353
GTCGACG150.002223065870.01070451
CCGGCGG800.065.6297121
CGCGTCT308.3880495E-658.318617
ATGCGCG308.412257E-658.2902644
TATGCGC252.4008259E-455.9495933
CGCCGTA1050.053.3544653
GGTCGAC200.006946317452.50802250
AGCGGGA200.006946317452.50802218
TCGTGAC200.00694854552.5037721
CCGACGG200.00694854552.5037721
GCGTGGC200.006950773352.499519
CGGATGG200.006961921752.4782429
GCCGTAT1100.050.93752354
GCCGGCG1100.050.91274320
GTCGCCG1400.050.0076451
TGGTCGC1500.048.99557549
Produced by FastQC (version 0.11.5)