FastQCFastQC Report
Thu 26 May 2016
SRR1781416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617613
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT17443528.243414565431753No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG137142.2204843486131285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC90611.467099947701878No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC23060.3733729698047159No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT17630.28545383597819346No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT16720.27071969016196223RNA PCR Primer, Index 36 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT11510.18636265752178144No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT11430.18506734799947538No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC11340.1836101247868811No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG8640.13989342840905228No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA150.002225415869.99206526
CGACACG150.002225415869.99206523
CGACTAA150.002225415869.99206548
AGGGGGG202850.067.7320370
TGCCGAC1250.064.392745
AAGGGGG220400.062.35454669
CGACTTC1100.060.44769348
ATGCCGA1400.059.99805544
ACGGGGG700.059.99319570
AAAGGGG232650.059.14652668
TGCCGTC226500.059.11471645
GCCGTCT226150.059.1133546
CCGTCTT225350.059.09026347
CGTCTTC226500.059.0374648
ATGCCGT227100.059.00953744
TATGCCG227350.058.94464543
CGTATGC229100.058.8858541
CTCGTAT229500.058.86424339
AAAAGGG234500.058.85967
GTATGCC228000.058.8581242