FastQCFastQC Report
Thu 26 May 2016
SRR1781415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251569
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22505489.46014811045876No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2830.11249398773298777No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670.10613390362087538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTAA150.002218015770.03863560
CGCTGGC207.9183665E-569.9968216
TCGGACG150.002223276569.9968211
GCCGGAG150.002223276569.9968254
GACAAAT150.002223276569.9968212
TTCTAAG150.002223276569.9968228
TGGCCGG150.002223276569.9968252
GGACGCT207.9183665E-569.9968213
ACAGCTG150.002223276569.996828
GCGGGTC150.002225032369.9828956
GAGTGTA252.838653E-669.9828934
TACGCAT207.9261765E-569.982895
TGCGGGT353.7052814E-969.982895
GTAGATG207.933993E-569.9689738
TATACGC207.933993E-569.968973
ATACGCA207.933993E-569.968974
CTTGTGC150.00222678969.968971
TGTGCGC207.933993E-569.968973
TTATACG252.8521026E-669.927242
TTGTGCG301.0284202E-769.927242
Produced by FastQC (version 0.11.5)