FastQCFastQC Report
Thu 26 May 2016
SRR1781415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251569
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT17696470.34412030099098No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG131965.245479371464688No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC82003.2595431074575965No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC27821.1058596249935406No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT21610.8590088603921787RNA PCR Primer, Index 36 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT21370.8494687342240101No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC16170.6427660005803576No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT13280.5278869813053277No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG12200.48495641354856917No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC7570.30091147955431713RNA PCR Primer, Index 36 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6760.2687135537367482No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC6150.2444657330593197No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG5390.21425533352678588No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5350.2126653124987578No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC4350.17291478679805541No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT4020.1597971133168236RNA PCR Primer, Index 36 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4010.1593996080598166No Hit
ACTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG3930.15621956600376039No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT3520.1399218504664724No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC3480.13833182943844433No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT3390.1347542821253811No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT3240.12879170327027575No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT2620.10414637733584026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA150.00221917470.02943438
ACTCACG150.00221917470.02943436
TATCTCG301.0180702E-770.02943436
AATATAG301.0180702E-770.02943436
TATAGTA207.900127E-570.02943438
ATATAGT207.900127E-570.02943437
ACTATCT150.00221917470.02943434
CTATCTC207.900127E-570.02943435
TAGAAAG150.002220925470.0155140
ACTGATA150.002220925470.0155153
CTTTAAA353.6907295E-970.0155157
CTCCTTG252.8308114E-670.0155154
CAACTAT150.002220925470.0155132
ACGTAAG150.002220925470.0155140
CACGAAA207.9079146E-570.0155139
GCTACAA150.002220925470.0155156
ATAGTAT252.8308114E-670.0155139
TGCTTTA353.6907295E-970.0155155
AACTATC150.002220925470.0155133
TCTCCTT207.9079146E-570.0155153
Produced by FastQC (version 0.11.5)