FastQCFastQC Report
Thu 26 May 2016
SRR1781414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172466
Sequences flagged as poor quality0
Sequence length76
%GC98

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15526290.02470052068233No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1770.10262892396182435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA150.002215737570.0473361
ATTAAAA150.002215737570.0473362
GTATCAT207.894438E-570.0269957
AGCAGGG150.002218291270.0269959
TATCATT150.002218291270.0269958
ATCATTA150.002218291270.0269959
TCATTAA150.002218291270.0269960
GTTGTGC150.00222340569.986371
TGCGGGT207.9171754E-569.986365
TCTCGGG252.8389186E-669.9660743
TTGTGCG207.928563E-569.9660642
GCGCTGG352.9650255E-759.9883115
TCGCCGT308.345303E-658.33889452
CGCCGTA308.345303E-658.33889453
CGCTGGG308.359677E-658.3219716
CTCGGGG308.374071E-658.30505844
CCGTATC252.3807748E-456.02160355
CGTATCA252.3807748E-456.02160356
GGCGCTG750.055.98909414
GCGCGTG252.3876154E-455.9890946
Produced by FastQC (version 0.11.5)