FastQCFastQC Report
Thu 26 May 2016
SRR1781414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172466
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT11605467.29094430206534Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG90125.225377755615599No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC46162.6764695650157133No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC21211.2298076142544037No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT21141.225748843250264Illumina PCR Primer Index 1 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC15630.9062655827815338Illumina PCR Primer Index 1 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT13310.7717463152157527Illumina PCR Primer Index 1 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG11280.6540419560956942No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT8900.516043741954936TruSeq Adapter, Index 1 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT8660.5021279556550277Illumina PCR Primer Index 1 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC7980.4626998944719539Illumina PCR Primer Index 1 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC6540.3792051766725036Illumina PCR Primer Index 1 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG5300.3073069474563102No Hit
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT4530.26266046641077084Illumina PCR Primer Index 1 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC4160.2412069625317454No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4050.23482889381095406Illumina PCR Primer Index 1 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG3550.20583767235281156No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT3310.19192188605290317No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3280.19018241276541462Illumina PCR Primer Index 1 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3180.18438416847378614Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT2680.15539294701564366No Hit
TCTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT2380.13799821414075816Illumina PCR Primer Index 1 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG2250.13046049656164113No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT2120.12292277898252409No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAGT2090.12118330569503555No Hit
GTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT1970.11422541254508134Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT1890.10958681711177856TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT1770.10262892396182435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT353.6816346E-970.016531
CTACACA150.002219609570.016531
ACTAAAG150.002219609570.0165350
ACAGATC150.002219609570.016536
ACGTCTA150.002219609570.0165347
TGCCGTA207.900302E-570.0165345
TAAAAAC301.0174699E-770.016533
GAAGTCT207.900302E-570.0165346
GAAGTCA252.8267877E-670.0165346
CTCTATA150.002219609570.016531
TTTTATA353.6816346E-970.016532
CCGTATT207.900302E-570.0165347
TGAAGTC353.6816346E-970.0165345
CTAATGA150.002219609570.0165351
CTAAAAC500.070.016531
AATACAA207.900302E-570.016533
GCCGTAT252.8267877E-670.0165346
TGCAGAC150.002219609570.0165345
AGTCATC252.8267877E-670.0165348
GTTATAC301.0174699E-770.016531
Produced by FastQC (version 0.11.5)