FastQCFastQC Report
Thu 26 May 2016
SRR1781413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346130
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20962660.562794325831334No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTT150.002223311570.0021717
GACGAGC150.002224587469.9920523
CGACGAG150.00222586469.98192622
ACGAGCG150.00222586469.98192624
CTAAGCT150.00222714169.971847
GGGATGC150.00222714169.97181
TGTGCGG401.364242E-1069.951583
CGCTGGC352.9692092E-760.00185816
TGCCGGC352.9692092E-760.00185819
TGGCGGC308.366214E-658.3351419
TGCGCGT308.394956E-658.3014035
CGTAAGC252.3746972E-456.06661666
CGCGTCT252.391742E-455.985547
TTGTGCG252.3985871E-455.953172
GTGCGGG1100.054.0613024
ACTTCGG200.00694709352.5016313
CGGGTGT200.00695504652.4864437
ACACGGA200.00695504652.4864436
GGTCGGC200.00695504652.48644350
GCGCGTC200.00695504652.4864436
Produced by FastQC (version 0.11.5)