FastQCFastQC Report
Thu 26 May 2016
SRR1781413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346130
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT16200346.80409094848756No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG124873.607604079392136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC75742.1881951867795335No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC27650.7988328084823622No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT20350.5879293906913587TruSeq Adapter, Index 25 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT17020.49172276312368185No Hit
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC14890.43018519053534804No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG10870.31404385635454884No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT10260.2964204200733828No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT8240.23806084419148874No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT7120.20570305954410195No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC6650.19212434634385925No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC5800.1675670990668246No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG5660.16352237598590125No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG4730.1366538583769104No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT4580.13232022650449254No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT3920.11325224626585387RNA PCR Primer, Index 25 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGACGT3890.1123855198913703No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT207.9199905E-570.0014546
CCATCGG150.002223404870.0014557
TACACGT207.9199905E-570.001455
CGAAAGC353.7034624E-970.0014441
ATACGAG150.002224679369.9913312
CCCGGCA150.002224679369.9913312
TAACTCG1200.067.0944136
AGGGGGG191900.066.8278570
GATAACT1350.064.82551634
GATGATC1250.064.4013331
CGACTTC600.064.1679948
ACTCGTA1050.063.3437938
AACTCGT1050.063.3437937
AAGGGGG208550.061.509369
TCGAAAG401.0628355E-861.26011740
ATGGGGG800.061.25126670
AAGCCGA401.0641088E-861.25126644
TCGTATC352.9664625E-760.00990740
CGTAAGC1400.060.00123641
GACCACG1750.059.9925718
Produced by FastQC (version 0.11.5)