FastQCFastQC Report
Thu 26 May 2016
SRR1781412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340655
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19459457.12348270244089No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG207.916576E-570.007222
CTTCGCC150.002222648170.00719512
CGAAGAT150.002225240569.98662625
GGACGGC252.3943673E-455.9728626
TACACGT252.3943673E-455.9728625
CGTTGTT200.00694502752.505413
ATTCCGG200.00694502752.505452
GACGAGC200.00694906552.49768423
TTCGGCA200.00695714552.4822632
GCCGTAT1000.049.0122454
CGTATCA1050.046.69891756
CGACGAA451.6810791E-646.67146722
GACGACA305.874816E-446.66460823
CACGTCT305.8833323E-446.6508987
CCGTATC1000.045.52474655
GGTCGCC1250.044.80460450
CGCCGTA1100.044.55003753
TGGTCGC1100.041.3678949
GTAGATC1700.041.1444338
GACTCCC350.00125388940.00411612
Produced by FastQC (version 0.11.5)