FastQCFastQC Report
Thu 26 May 2016
SRR1781412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340655
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT15279944.85447153278243No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG121093.554622712128106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC77022.2609384861516784No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT18550.544539196547827No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC18440.5413101231451175No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT14500.4256505849026141Illumina PCR Primer Index 6 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT11430.3355300817542675No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC10900.31997181899575816No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC7930.23278683712260204No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG7100.20842201053852139No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5520.1620407743905124No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC5140.1508857935447887No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT4280.12564031057815092No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4240.12446610206807475No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC4180.12270478930296047No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4100.12035637228280811No Hit
ACTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG3450.10127548399407026No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG207.9151905E-570.009740
TCGAAAG252.8343693E-670.0096940
CGAAATA150.00222363169.9994148
CCTACGG207.920949E-569.9994112
AATGACG353.7034624E-969.9994143
CTACGGT207.920949E-569.9994113
ACGGTGA207.920949E-569.9994115
GACAACG455.456968E-1269.98913618
AGGGGGG179000.067.7703870
TCACCGA1150.066.95595611
CACGTAT900.066.1202639
AATGCCG900.066.1105643
TCGAATG1600.065.6340940
CGAATGC1200.064.17554541
ATCACCG1200.064.1661310
AATATCG1100.063.64517236
TATGACG1550.063.22527743
CTCGAAT1400.062.5086539
AAGGGGG194700.062.35951669
ATGACGA453.8380676E-1062.221744
Produced by FastQC (version 0.11.5)