FastQCFastQC Report
Thu 26 May 2016
SRR1781411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270789
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17612365.0406774278128No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC150.002221359670.01350416
CGTCACC150.002221359670.01350412
CCTTATG150.0022229970.0005510
TTGTACG150.002226252869.974682
GTTGGAC150.002226252869.974683
CTGCGGG252.3876637E-456.00044318
TATAGGG252.392031E-455.9797433
TGACGCG200.00694102152.51012413
CGCCGGA200.00694102152.51012453
GTGACGC200.00694102152.51012412
CCCTTAT200.006951181752.4907079
CGCCCTA200.006951181752.49070727
GATTCCC200.006956266252.48100737
ATCTCCC200.00696135452.4713143
GACGAAC305.8765116E-446.6584123
TGCGGGT305.88187E-446.6497845
CTCTTAC350.00125515939.992921
GATTGCC350.001256300839.9855277
GTCGCCG400.002413888535.00675251
GACGCGG400.002413888535.00675214
Produced by FastQC (version 0.11.5)