FastQCFastQC Report
Thu 26 May 2016
SRR1781411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270789
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT13762350.82296548234972No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG107863.983175092045836No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC68382.5252133580019867No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC19600.7238107899508474No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT13420.4955888163847128Illumina PCR Primer Index 7 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT13130.48487937102319517No Hit
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC8760.32349910816170524No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT7500.27696841452200793No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG6310.2330227594178493No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG4790.17689049407472238No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC4570.16876608724874348No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT4150.15325585603551103No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT3900.14402357555144413No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3790.13996137213845466No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3710.13700704238355327No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT3320.12260468482840883No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT3290.1214968111703208No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC2880.10635587117645104No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT2790.10303225020218694No Hit
ACTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG2720.1004472116666482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA301.018434E-770.0286432
GGTCAAC700.070.0286433
CGCTCAT207.901659E-570.0286432
CGTAGGT150.002219457970.0286435
CAAATCG150.002219457970.0286436
ACTCGTA550.070.0286438
AACTCGT500.070.0286437
CGGTCAA1150.070.0286432
CAACTCG750.070.0286436
TCGAAAG207.901659E-570.0286440
TAATCCG150.002222713370.0027743
CGACATA150.002224342469.98984548
GCCGACA301.022363E-769.98984546
ACGTCTA150.002224342469.98984547
CCGACAT301.022363E-769.98984547
ATACGAG150.002224342469.98984512
TATACGA150.002224342469.98984511
AGGGGGG159200.068.231370
TCGTAAG950.066.3429240
TACGAGC950.066.3061713
Produced by FastQC (version 0.11.5)