FastQCFastQC Report
Thu 26 May 2016
SRR1781410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514148
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27236152.97326839742642No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG107932.099201008269992No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT11210.21803060597337728No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11180.2174471163945012No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7600.14781735998195072No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6920.13459159619409197No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6620.12875670040533077No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6000.11669791577522426No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5820.11319697830196751No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG5330.10366664851365756No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5330.10366664851365756No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCT150.002215810670.0667439
CGTATCG301.019871E-770.0326256
TATCGTT207.91381E-570.0189958
ACTCCGC150.002222668670.0121769
CGTTAAG150.00222352770.00535661
GAACGTA150.002224385769.998541
GGATACG150.002229542569.957692
CGAAATT150.00223040369.9508810
GCGTTTC150.00223040369.9508822
GAGGTAC150.00223040369.9508826
TTTTGCG150.00223040369.9508819
TCGACGG150.00223040369.9508818
ACGTACC150.002231263669.9440843
CCGACGG950.066.2692621
TACGCGT501.4551915E-1162.9557885
CGCGTCT1450.062.7145847
TTGTGCG1300.061.885642
ACGCGTC650.059.1892056
GCCGAAT601.8189894E-1258.37188754
CGAACGT308.408437E-658.292425
Produced by FastQC (version 0.11.5)