FastQCFastQC Report
Thu 26 May 2016
SRR1781410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514148
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT16299931.7027392890763No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG395517.69253211137649No Hit
ATTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT194853.7897648148004075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186463.626582229241386No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC134952.6247306223110853No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC69851.3585582361499025No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT38870.7560079976971611RNA PCR Primer, Index 17 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT21130.41097116005508144No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT21020.40883169826586896No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC19860.3862701012159923No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT19750.3841306394267799No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12820.24934454670639583No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG11990.2332013350241565No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11880.231061873234944No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC11140.21666913028933305No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT10690.2079167866061912No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC10390.20208189081742997No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9540.18554968608260658No Hit
CTTATACACATCTCCGAGCCAACGAGACAAATACAGATCTCGTATGCCGT9240.17971479029384535No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT8960.17426888755766823RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGA8440.16415506819048212No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGACGT8020.1559862140862164No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGAATGCCGT7870.15306876619183582No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC7110.13828703019364072No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCAGT7070.1375090440884726No Hit
CTTATACACATCTCCGAGACCACGAGACAAATACAGATCTCGTATGCCGT7030.13673105798330443No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT6470.12583925251095016No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT6390.12428328030061384No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG6290.12233831503769342No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTAAGCCGT6060.1178648949329765No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT6020.11708690882780834No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5910.11494744703859588No Hit
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5620.10930704777612672No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT5500.10697308946062223No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT150.00222325970.0074932
TTATACG207.924073E-570.000682
ATACGCA207.924073E-570.000684
TATACGA207.924073E-570.0006811
CGTATCA207.927892E-569.9938741
TATGAGC150.00222497569.9938748
CTCGCTT150.00222497569.9938750
GACGAGT150.00222497569.9938741
TTTTCGT150.00222497569.9938743
ATCGCAT150.00222497569.9938754
GTCGGTT150.00222497569.9938724
AGGGGGG233400.066.10818570
ATTATAC35950.065.132761
TCTTTAT9100.062.692921
GAGACTA1350.062.21677424
TACGAGA352.9705188E-760.0064213
CGAGACT1350.059.6244123
TCGAATG6100.059.09318540
CGAATGC5400.058.9763241
CGTAAGA950.058.94220741
Produced by FastQC (version 0.11.5)