FastQCFastQC Report
Thu 26 May 2016
SRR1781409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640184
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17534227.38931307249166No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG128792.011765367456856No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT11160.174324881596541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCAT150.002223281270.009348
CTCGCAT150.002223970470.0038257
GCGGCCT150.002223970470.0038257
CGGCGTT150.002224659569.9983661
TTTCGCA150.002224659569.9983632
ATGCACG353.7161954E-969.987424
TATGCGC352.9775765E-759.989223
TTATGCG308.373152E-658.336522
CGTATCA21450.056.7907656
TCGCCGT21150.056.7687752
CGCCGTA21700.056.62042653
CCGTATC21300.056.5333355
GGTCGCC21850.056.39192650
TCTCGGT21700.056.29780243
CTCGGTG22150.055.94422544
ATCTCGG21650.055.94276442
GTCGCCG22050.055.8804451
GCCGTAT21850.055.43070254
TGGTCGC21950.055.33764349
CGGTGGT22650.055.3274446
Produced by FastQC (version 0.11.5)